产品货号:
Z37017
中文名称:
PCNA抗体
英文名称:
Anti-PCNA Antibody (Clone#2G2)
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-PCNA Antibody (Clone#2G2) |
指标别名 | PCNA |
克隆性 | Monoclonal |
检验物种 | human,mouse,rat |
应用范围 | WB,IHC,ICC/IF,FCM |
基因名称 | PCNA |
克隆号 | 2G2 |
抗体来源 | Mouse |
抗体类型 | IgG2b |
免疫原 | E.coli-derived human PCNA recombinant protein (Position:M1-S261). |
实际分子量 | 36KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | protein G purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication.It is mapped to 20p12.3.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
Uniprot ID | P12004 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
基因名全称 | proliferating cell nuclear antigen |
蛋白名全称 | Proliferating cell nuclear antigen |
推荐稀释比 | Western blot(WB) 1:500~2000 Immunohistochemistry in paraffin section (IHC) 1:50~400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400 Flow cytometry (FCM) 1~3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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